Abstract: Kornerupine is a complex hydrous magnesia-iron-aluminum borosilicate that ranges in colour from colourless to yellow, green, brown and blue, etc.The gem-grade green kornerupine is the most precious and popular in the gemstone market now. Nonetheless, due to its rarity, the information about the origins and gemmological characteristics of the gem-quality green kornerupine is little known. In this paper, the gemmological and mineralogical characteristics of the kornerupine samples from Tanzania, Myanmar, and Sri Lanka respectively were studied by conventional gemmological methods, UV-Vis-NIR, Raman spectroscopy, EMPA and LA-ICP-MS. Kornerupine samples from the three origins exhibited similar gemmological characteristics, except for the inert ultraviolet fluorescence response of the Myanmar samples, which is attributed to higher iron content. Besides, all the kornerupine samples from the three origins displayed abundant fluid inclusions, needle-like inclusions and graphite inclusions with varying degrees of crystallization, indicating their metamorphic origin. The B element apfu (atoms per formula unit) serves as a crucial criterion for the classification of kornerupine mineral species. The testing results of B element using LA-ICP-MS indicate that kornerupine samples from Myanmar are predominantly classified as kornerupine, whereas those samples from Tanzania and Sri Lanka are primarily identified as prismatine. Due to differences of trace element compositions, the gemmological properties of kornerupine samples from Myanmar were different compared to the other two origins. The V-Cr-Mn and Fe-Mn-V are trace elements "fingerprints" that provide a useful tool for identifying the provenance of kornerupines from Tanzania, Myanmar, and Sri Lanka. V³⁺ is the predominant colouring element of kornerupines from Tanzania, and Sri Lanka, giving rise to more yellowish green colour. Chromium is the predominant chromophore of kornerupine from Myanmar, according to the combined observations from UV-Vis-NIR spectroscopy and trace element analyses.