Abstract: Surface microtopography,representation and tracing of nacreous layers of Pteria penguin and blister pearl from Sanya, Hainan Province, are studied by using interference microscopeand environmental scanning electron microscope and atomic force microscope. The research indicates that the microscale spiral (vortex) growth dislocation is another important growth mechanism of Pteria penguin and blister pearl from Sanya, Hainan Province. It indicates that the microscale spiral (vortex) growth dislocation is related to the ecosystem dynamics from South China Sea. The research present situation and advancement of growth structure of nacreous layer are discussed.