Abstract: Mortise and tenon structure as a traditional structural technology can be combined with the structure of jewelry, thus jewelry can be wore multi-ways. Therefore, mortise and tenon structure has a certain application value in jewelry design. In this paper, the authors achieved a small volume, high-precision structure printing based on 3D printing technology and explored the design and application of mortise and tenon structure in 3D printing jewelry. This paper also analyzed the basic situation of mortise and tenon structure and 3D printing technology, the transformation relationship among mortise and tenon structure, jewelry design and 3D printing technology, and the applicability of mortise and tenon structure and 3D printing technology in jewelry design. Through 3D printing technology, the authors obtained an innovative application design scheme of replaceable mortise and tenon structure jewelry. This paper summarized the transformation mode of mortise and tenon structure in jewelry structure and the design focus of the structure, material, size etc. which has certain reference significance for the application of mortise and tenon structure in 3D printed jewelry design, and provides thinking for the structural innovation and material replacement of jewelry design.