Abstract: In this study, we collected and characterized the amber fluorescent behaviors by obtaining the general amber varieties 3D-fluorescence spectra from five localities including Baltic Sea region, Dominican Republic, Mexico, Myanmar and Fushun, China. These spectral features show that amber from Baltic Sea region has the strongest emission peak with the center near 435 nm in blue region, which is best excited by 350 nm light. Dominican amber emit three typical narrow peaks at 445、474 nm and 508 nm. They are optimally excited by 440 nm light, closcly followed by, 415 nm light. The strongest fluorescence center of Mexican amber is unfixed but nomally between 420~465 nm range. Burmese and Fushun amber behave similarly. Their luminescence centers appear in visible and ultraviolet region. Burmese amber has a strong 426 nm emission band excited by 375 nm light, while Fushun amber has the strongest emission peak with the center near 414 nm and is best excited by 365 nm light. In ultraviolet region, Burmese amber occur two weak emission peaks near 334 nm and 347 nm by 295 nm light excitation. Fushun amber also have two weak centers, but these weak centers are near 360 nm and 399 nm and are excited by 290 nm light. On the other hand, when all samples were excited by 375 nm light, the wavelength of their strongest emission peaks shows the following order: Baltic Sea region > Dominican Republic > Mexico > Myanmar ≈ Fushun, China.