Abstract: Chinese national standard on cultured pearls can't stand for the method and standard of the total quality of jewelry pearls, which limits its application in the market. This paper takes Chinese freshwater non-nucleus cultured pearl chains as examples, use the market survey to collect freshwater pearl price information in China, analyzes quality factors including colour, luster, size, finish, and shape, make sure each quality factor's weight that impacts the price of freshwater pearls, and determine the weight coefficient. The total quality level of the pearl samples was determined by weight method and luster dominant method. The results show that based on the weight method, the influence coefficients of the three main quality factors are determined, which is of certain reference significance. However, due to the inability to collect all grade data of factors that affect the quality of freshwater pearls in the market, it is difficult to make accurate quantitative analysis, and it is inevitable to have errors in the weight determination. Determine the total quality level based on luster, depending on the weight and the standard of coloured gemstones, is not scientific enough, and the practical implementation of Chinese national standards in the application of the market also needs constant investigation and study.